In Vitro ADME Assays In Vitro ADME assay is an essential step in drug development. We will help you to understand the Adsorption, Distribution, Metabolism, Excretion (ADME) as well as the Pharmacokinetics (PK) of your compounds, which will help both your candidate selection and knowledge of possible drug interactions. Our in vitro ADME/PK studies include…
One of our clients was developing an inhalation formulation containing two actives at extremely low concentration. One active was a lypopeptide with no chromophore, the other active was a phosphorothioate oligonucleotide with very unusual properties. The client turned to us after two other CROs failed to develop a viable assay, and thus critical animal studies were significantly delayed. We were able to develop suitable assays in a short timeframe and transfer the methods to the animal facility. Drug development program has since progressed to the clinical stage.
One of our clients requested to develop a method for a very low abundance plant intermediate. Major obstacles were instability of this metabolite during sample preparation and its surfactant nature, which presented a significant challenge in its chromatographic separation. We were able not only successfully develop a very sensitive LC-MS/MS method, but also achieve much higher recoveries in our spiking experiments compared to the best values published previously (90% vs 15%).
We received a request through the website to perform an urgent investigation of a plant culture media to verify concentration of two carbohydrates and an antibiotic at a very low concentration. In addition, antibiotic did not have any UV absorbance, thus complicating its detection. Nevertheless, only two days later, a suitable LC-MS method was developed and successfully used to troubleshoot media preparation. Our client was able to uncover complex interactions between different media components and come up with a solution for a viable formulation.
We are continuing our blog with a review of cell death assays for drug discovery. In this posting I will describe cellular metabolism assays to use in your project to measure cell death, cytotoxicity, and proliferation. All assays in our blog are available as a service from Alera Labs. Please contact us at (919) 228-8209…
We are continuing our blog with a review of cell death assays for drug discovery. In this posting I will describe the most simple and useful assays to use in your project to measure cell death. All assays in our blog are available as a service from Alera Labs. Introduction As we have discussed in…
We are starting our blog with a review of cell death assays for drug discovery. In this posting I will describe different modes of cell death as well as background information necessary for better understanding of cell death assays. All assays in our blog are available as a service from Alera Labs. Please contact us…
