One of our clients was developing an inhalation formulation containing two actives at extremely low concentration. One active was a lypopeptide with no chromophore, the other active was a phosphorothioate oligonucleotide with very unusual properties. The client turned to us after two other CROs failed to develop a viable assay, and thus critical animal studies were significantly delayed. We were able to develop suitable assays in a short timeframe and transfer the methods to the animal facility. Drug development program has since progressed to the clinical stage.
One of our clients requested to develop a method for a very low abundance plant intermediate. Major obstacles were instability of this metabolite during sample preparation and its surfactant nature, which presented a significant challenge in its chromatographic separation. We were able not only successfully develop a very sensitive LC-MS/MS method, but also achieve much higher recoveries in our spiking experiments compared to the best values published previously (90% vs 15%).
We received a request through the website to perform an urgent investigation of a plant culture media to verify concentration of two carbohydrates and an antibiotic at a very low concentration. In addition, antibiotic did not have any UV absorbance, thus complicating its detection. Nevertheless, only two days later, a suitable LC-MS method was developed and successfully used to troubleshoot media preparation. Our client was able to uncover complex interactions between different media components and come up with a solution for a viable formulation.