LC/MS

Liquid Chromatography/Mass Spectrometry (LC/MS) refers to the combination of liquid chromatographic separation with mass spectrometric detection.  The combination of these two powerful techniques gives the chemical analyst the ability to separate, detect and identify non-volatile compounds (small molecules) and biologically active compounds (large molecules) such as proteins.  Mass spectrometry is capable of providing structure, molecular weight, empirical formula, and quantitative information about a specific analyte.  The two API methods available are Electrospray Ionization (ESI) and Atmospheric Pressure Chemical Ionization (APCI). The result is a quantitative analysis and mass or fragmentation spectrum for each component.

Instrument Capabilities

Agilent(3) featuring:

Mass range 10 to 4000 Da

ESI (Electrospray Ionization)

APCI (Amtosphereic Pressure Chemical Ionization)

Syringe Pump

Standard and Well Plate autosamplers

Fast, scan-to-scan, polarity switching for both positive and negative ions in a single analysis

Range of Applications

• Assay and related substances
• Forced degradation studies
• Characterization and identification of contaminants
• Stability indicating assays
• Filter compatibility screening
• Method transfer
• Reference standard qualification
• Routine container/closure testing
• Formulation compatibility and stability
• Degradation product identification and profiling
• Drug-excipient interaction studies
• Deformulation of products
• Identification of packaging extractables and leachables
• Peak purity verifications
• Identification of unknown impurities
• Identification and characterization of reaction intermediates and by-products
• Patent litigation support